Both autologous and allogeneic cells are obtained from donors utilizing methods including, but not limited to, apheresis, aspirates, and surgical biopsies. The donor cells of interest are processed to remove undesirable contaminants and prepared for cell culture procedures.
To increase the number required for the final cell product, the donor cells are cultured in culture media supplemented with cytokines, growth factors, and essential amino acids and vitamins. Bioreactor systems designed for attachment-dependent donor cells are also used.
Many donor cells are not amenable to culture systems that exclude the use of animal sera or components of non-animal origin. If modification of the donor cells is required, VWR, part of Avantor provides a line of transfection reagents proven to meet your needs.