Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
Provides a simple and versatile method for protein extraction from adherent or suspended Sf9 and Sf21 insect cells
Compatible with electrophoresis and chromatographic applications
Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis