The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
- For generation of cytosolic and ultra pure nuclear fractions
- Provided with nuclear protein solubilisation buffers
This kit is based on organic buffers and contains a proprietary combination of various salts and agents. The kit is provided with reagents for solubilisation of nuclear fraction.
The kit is designed for >50 preps, depending on the tissue used, where one prep is 20×10⁶ mammalian cells or 100 mg mammalian tissue.