rmp Protein A Sepharose™ Fast Flow is a low leakage, non-mammalian based affinity medium designed for high purity separation of monoclonal and polyclonal antibodies at the laboratory and process scales.
- Multi-point attachment of rProtein A by chemically-stable amide bonds
- Coupling chemistry that minimises leakage of protein A ligand
- Stable in aqueous buffers commonly used in protein A chromatography – 10 mM HCl (pH 2), 1 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 6 M GuHCl, 20% ethanol
rmp Protein A Sepharose™ Fast Flow is composed of recombinant protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix by reductive amination resulting in multi-point attachment. Reductive amination creates chemically stable amide bonds and resulting low ligand leakage.
rmp Protein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.
As a member of the BioProcess resins range, rmp Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Ligand: Recombinant protein A (E.coli)
Matrix: Cross-linked agarose, 4%, spherical
Binding capacity: >Min. 22 mg human IgG/ml