Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.
- Bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for western blotting
- Effective with IP assays performed using protein A, protein G, or anti-IgG agarose beads and any blocking buffer
- Eliminates the extra work associated with covalently immobilizing IP antibodies as a means of overcoming western blot interference
- Horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
- Clear western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands
The Clean-Blot™ IP reagent allows trouble-free western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot™ IP reagent and kit eliminate detection-interference from both heavy-chain (approx. 50 kDa) and light-chain (25 kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.
Informations de livraison: Kit contains Clean-Blot™ Detection Reagent (HRP), blocking buffer and Pierce™ ECL Substrate (chemiluminescent).
