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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Fournisseur: Biotium
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30027.EA 491 EUR
30027.
Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells
Tests Tests cellulaires
The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

  • Detect live and dead bacteria cells in a cell population simultaneously
  • Rapid (15 minute) dye loading and measure without washing
  • Perform viability and cytotoxicity assays at the same time
  • Analysis compatible with flow cytometers and fluorescence microscopes using popular settings for fluorescein and propidium iodide

DMAO is a green-fluorescent nucleic acid dye which stains both live and dead bacteria with intact and damaged cell membranes. EthD-III is a red-fluorescent nucleic acid dye that only stains dead bacteria with damaged cell membranes. With an appropriate mixture of DMAO and EthD-III, bacteria with intact cell membranes stain fluorescent green, whereas bacteria with damaged cell membranes stain fluorescent red. The assay principles are general and applicable to most bacteria types.

Informations de livraison: Kit contains 2 vials DMAO, 5 mM (100 µl/vial) and 2 vials (150 µl/vial): Ethidium Homodimer-III (EthD-III), 2 mM (150 µl/vial).
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