Imprimer

EDTA buffer, pH 8.5, 10×, antigen retriever, Sigma-Aldrich®

Fournisseur: Merck
Ratings: Ecrire un avis

Total Evaluation: 0
Moyenne des Notes : 0.0 sur 5

E1161-100MLEA 34.2 EUR
E1161-100ML
EDTA buffer, pH 8.5, 10×, antigen retriever, Sigma-Aldrich®
Tampons Tampons pour la purification d'anticorps
Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.

In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8.5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualize antigens by immunohistochemical analysis.
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