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Phénol ≥99.0% pour les biotechnologies pH 4.5

Marques : VWR Chemicals
Ratings: Ecrire un avis

Total Evaluation: 0
Moyenne des Notes : 0.0 sur 5

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Synonyme(s): Acide phénique

0981-400MLEA 256 EUR
0981-400ML 0981-100ML
Phénol ≥99.0% pour les biotechnologies pH 4.5
Phénol

Une alternative idéale pour le phénol saturé en eau dans de nombreux protocoles. Elle offre l'extrême pureté et les performances du phénol saturé en eau avec une stabilité et une sécurité grandement accrues.


  • Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
  • Liquid phenols offer a safer, easier alternative to crystalline phenol
  • Optimised pH for the purification of RNA
  • Long term stability > one year


For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.

Formule: C₆H₅OH
Poids moléculaire: 94,11 g/mol
Point d'ébullition: 182 °C
Température de stockage: Température ambiante
Numéro MDL: MFCD00002143
Numéro CAS: 108-95-2
EINECS: 203-632-7
UN: 2821
ADR: 6.1,II

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Résultats des tests de spécification

O.D.@ 330 nm ≤0.2
O.D.@ 405 nm ≤0.1
O.D.@ 510 nm ≤0.1
pH (Tampon) @25°C 4.1 – 4.5
Pureté ≥99 %

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